Hotspot

petN-psbM

LSC · 28307-29192 · 886 bp

Back to Strobilanthes

Pi 0.0133
MarkerSeek score 68.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
886
variable_sites
43
pi
0.0133
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 982-1057 bp 1.0 77.9
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 980-1055 bp 1.0 77.9
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 978-1053 bp 1.0 77.3
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 976-1051 bp 1.0 77.3
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG TCATTCTAGTTCCTACCGCCT 55.0 47.6 57.9 58.0 979-1054 bp 1.0 77.3