Hotspot

trnC-GCA-petN

LSC · 27388-28216 · 829 bp

Back to Strobilanthes

Pi 0.0294

MarkerSeek score 81.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 81
pi: 0.0294
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	939-1125 bp	1.0	89.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	937-1123 bp	1.0	89.4
trnC-GCA-petN_p3	GAGAATTTTGGCGACATGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	990-1176 bp	1.0	89.1
trnC-GCA-petN_p4	GAGAATTTTGGCGACATGGC	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	988-1174 bp	1.0	89.1
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	940-1126 bp	1.0	88.8