Hotspot

trnS-GCU-trnS-CGA

LSC · 7083-7989 · 907 bp

Back to Strobilanthes

Pi 0.0376

MarkerSeek score 80.70

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 94
pi: 0.0376
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	ACCACTAAACTACACCCGCT	50.0	50.0	58.0	58.7	820-1053 bp	1.0	86.6
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	ACCACTAAACTACACCCGCT	50.0	50.0	58.0	58.7	819-1052 bp	1.0	86.6
trnS-GCU-trnS-CGA_p3	AGCAATCCGACGCTTTAGTC	ACCACTAAACTACACCCGCT	50.0	50.0	58.4	58.7	822-1055 bp	1.0	83.8
trnS-GCU-trnS-CGA_p4	CGACGCTTTAGTCCACTCAG	ACCACTAAACTACACCCGCT	55.0	50.0	58.4	58.7	815-1048 bp	1.0	83.6
trnS-GCU-trnS-CGA_p5	CAATCCGACGCTTTAGTCCA	TACCACTAAACTACACCCGC	50.0	50.0	58.0	57.0	821-1054 bp	1.0	83.6