Hotspot

psbK-psbI

LSC · 6383-6793 · 411 bp

Back to Strobilanthes

Pi 0.0366
MarkerSeek score 75.80
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
411
variable_sites
35
pi
0.0366
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG CGAAGAGTTTCAGGGTAAGCA 50.0 47.6 57.9 58.0 448-467 bp 1.0 81.4
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG ACGAAGAGTTTCAGGGTAAGC 50.0 47.6 57.9 58.0 449-468 bp 1.0 81.4
psbK-psbI_p3 CTTTTGTTTGGCAAGCTGCT CGAAGAGTTTCAGGGTAAGCA 45.0 47.6 58.1 58.0 453-472 bp 1.0 81.3
psbK-psbI_p4 CTTTTGTTTGGCAAGCTGCT ACGAAGAGTTTCAGGGTAAGC 45.0 47.6 58.1 58.0 454-473 bp 1.0 81.3
psbK-psbI_p5 TTTTGTTTGGCAAGCTGCTG CGAAGAGTTTCAGGGTAAGCA 45.0 47.6 58.3 58.0 452-471 bp 1.0 79.0