Hotspot

rps16-trnQ-UUG

LSC · 4618-5743 · 1126 bp

Back to Strobilanthes

Pi 0.0248
MarkerSeek score 73.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1126
variable_sites
81
pi
0.0248
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 995-1241 bp 1.0 87.8
rps16-trnQ-UUG_p2 TTCTGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1006-1252 bp 1.0 87.7
rps16-trnQ-UUG_p3 TTCTGTCCTTCAAGTCGCAC TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.1 57.3 1010-1256 bp 1.0 83.0
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 999-1245 bp 1.0 83.0
rps16-trnQ-UUG_p5 TTCTGTCCTTCAAGTCGCAC TAAGGCAACGGGTTTTGGTC 50.0 50.0 58.1 58.7 1036-1282 bp 1.0 82.8