Hotspot

rps4-trnT-ACA

LSC · 44704-45094 · 391 bp

Back to Strigosella

Pi 0.0179

MarkerSeek score 67.20

Primer available yes

Label rps4-trnT-ACA

Feature Metadata

label_name: rps4-trnT-ACA
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 13
pi: 0.0179
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-ACA_p1	CGGAGCGTGATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	569-577 bp	1.0	81.0
rps4-trnT-ACA_p2	CGGAGCGTGATTGGTTTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	537-545 bp	1.0	80.3
rps4-trnT-ACA_p3	TCTAAGATCGCTTCCTGCCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	553-561 bp	1.0	79.3
rps4-trnT-ACA_p4	TCTAAGATCGCTTCCTGCCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.2	57.8	521-529 bp	1.0	78.6
rps4-trnT-ACA_p5	CGGAGCGTGATTGGTTTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	577-585 bp	1.0	78.0