Hotspot

psbC-trnS-UCA

LSC · 34135-34315 · 181 bp

Back to Strigosella

Pi 0.0343

MarkerSeek score 61.90

Primer available yes

Label psbC-trnS-UCA

Feature Metadata

label_name: psbC-trnS-UCA
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 12
pi: 0.0343
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UCA_p1	GAGCGACGTTCTGCAGAATA	ACGGGTATACTGGATCGTGT	50.0	50.0	58.1	57.9	695-696 bp	1.0	80.5
psbC-trnS-UCA_p2	ACCGCTTACCCTAGTGAGTT	TGGGAGGGGTCATCTCATTT	50.0	50.0	58.1	58.0	1396-1402 bp	1.0	80.4
psbC-trnS-UCA_p3	GAGCGACGTTCTGCAGAATA	TGGGAGGGGTCATCTCATTT	50.0	50.0	58.1	58.0	1114-1120 bp	1.0	80.4
psbC-trnS-UCA_p4	ACCGCTTACCCTAGTGAGTT	ACGGGTATACTGGATCGTGT	50.0	50.0	58.1	57.9	977-978 bp	1.0	80.2
psbC-trnS-UCA_p5	TATGGTCTGGGGAGGCTTAC	TGGGAGGGGTCATCTCATTT	55.0	50.0	58.2	58.0	1485-1491 bp	1.0	79.3