Hotspot

rpoB-trnC-UGC

LSC · 25241-26334 · 1094 bp

Back to Strigosella

Pi 0.0107

MarkerSeek score 65.40

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 22
pi: 0.0107
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1176-1182 bp	1.0	80.2
rpoB-trnC-UGC_p2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1158-1164 bp	1.0	78.1
rpoB-trnC-UGC_p3	GTTCCCTCTTTTCCATCCCC	TCAAGTCTGTTGTTGAGGCG	55.0	50.0	57.9	58.4	1200-1206 bp	1.0	77.5
rpoB-trnC-UGC_p4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.1	58.1	1177-1183 bp	1.0	74.2
rpoB-trnC-UGC_p5	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	55.0	52.6	57.9	58.0	1198-1204 bp	1.0	72.8