Hotspot

trnS-AGC-trnG-GGA

LSC · 6913-7543 · 631 bp

Back to Strigosella

Pi 0.0104

MarkerSeek score 61.40

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 13
pi: 0.0104
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	50.0	50.0	57.8	57.2	741-755 bp	1.0	75.6
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	50.0	50.0	58.2	57.2	739-753 bp	1.0	75.3
trnS-AGC-trnG-GGA_p3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	50.0	50.0	57.6	57.2	736-750 bp	1.0	73.6
trnS-AGC-trnG-GGA_p4	TCCAACGCTTTAGTCCACTC	TGAATCAAACCGAGAGACCC	50.0	50.0	57.5	57.2	742-756 bp	1.0	73.3
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	GAATCAAACCGAGAGACCCT	50.0	50.0	57.8	56.9	740-754 bp	1.0	73.2