Hotspot

trnK-AAA-trnQ-CAA

LSC · 4352-5705 · 1354 bp

Back to Strigosella

Pi 0.108

MarkerSeek score 85.70

Primer available yes

Label trnK-AAA-trnQ-CAA

Feature Metadata

label_name: trnK-AAA-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 1354
variable_sites: 248
pi: 0.108
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-trnQ-CAA_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1412-2185 bp	1.0	90.0
trnK-AAA-trnQ-CAA_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1411-2184 bp	1.0	90.0
trnK-AAA-trnQ-CAA_p3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1398-2171 bp	1.0	87.7
trnK-AAA-trnQ-CAA_p4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	1423-2196 bp	1.0	86.8
trnK-AAA-trnQ-CAA_p5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	1421-2194 bp	1.0	85.6