Hotspot

trnH-CAC-psbA

LSC · 77-392 · 316 bp

Back to Strigosella

Pi 0.0177

MarkerSeek score 70.20

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 316
variable_sites: 11
pi: 0.0177
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	350-374 bp	1.0	84.9
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	365-389 bp	1.0	84.7
trnH-CAC-psbA_p3	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	351-375 bp	1.0	84.7
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	368-392 bp	1.0	84.6
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	366-390 bp	1.0	84.5