Hotspot

orf490

Genome · 102-1574 · 1473 bp

Back to Streptosarcina

Pi 0.436

MarkerSeek score 81.30

Primer available yes

Label orf490

Feature Metadata

label_name: orf490
feature_type: gene
region: Genome
length_bp: 1473
variable_sites: 626
pi: 0.436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf490_p1	GGGGACCGATCTAAGGAAGA	TGTTCAAAGGCCTCTTGGTC	55.0	50.0	57.9	58.0	1973 bp	0.5	59.2
orf490_p2	GGGGACCGATCTAAGGAAGA	GGCCTCTTGGTCTATTGCTC	55.0	55.0	57.9	58.0	1965 bp	0.5	59.0
orf490_p3	GTGTTTCCTTCTTTGCAGGC	TGTTCAAAGGCCTCTTGGTC	50.0	50.0	57.9	58.0	1953 bp	0.5	58.7
orf490_p4	GGGGACCGATCTAAGGAAGA	GGGCTTGTCGAACATACTGT	55.0	50.0	57.9	57.9	1851 bp	0.5	58.6
orf490_p5	GTGTTTCCTTCTTTGCAGGC	GGCCTCTTGGTCTATTGCTC	50.0	55.0	57.9	58.0	1945 bp	0.5	58.6