Hotspot

orf340

Genome · 98436-99458 · 1023 bp

Back to Streptosarcina

Pi 0.414

MarkerSeek score 79.00

Primer available yes

Label orf340

Feature Metadata

label_name: orf340
feature_type: gene
region: Genome
length_bp: 1023
variable_sites: 423
pi: 0.414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf340_p1	ATTGCTCGTAGGCTGTTCTG	AGCGTACCGACAACTTCATC	50.0	50.0	58.0	58.0	1785 bp	0.5	59.8
orf340_p2	CATTGCTCGTAGGCTGTTCT	AGCGTACCGACAACTTCATC	50.0	50.0	58.0	58.0	1786 bp	0.5	59.8
orf340_p3	ATTGCTCGTAGGCTGTTCTG	GGCTTGCGACTCACAATCTA	50.0	50.0	58.0	58.0	2022 bp	0.5	59.8
orf340_p4	CATTGCTCGTAGGCTGTTCT	GGCTTGCGACTCACAATCTA	50.0	50.0	58.0	58.0	2023 bp	0.5	59.8
orf340_p5	CCAAGCTCCCTCATACACAC	AGCGTACCGACAACTTCATC	55.0	50.0	58.0	58.0	1909 bp	0.5	59.7