Hotspot

trnF(gaa)-rpl19

Genome · 163394-166546 · 3153 bp

Back to Streptosarcina

Pi 0.275

MarkerSeek score 57.70

Primer available yes

Label trnF(gaa)-rpl19

Feature Metadata

label_name: trnF(gaa)-rpl19
feature_type: igs
region: Genome
length_bp: 3153
variable_sites: 22
pi: 0.275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.03
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(gaa)-rpl19_p1	AGCCAATTGATAGCAGAGGC	TAAACCCCTTTGGCTCAACC	50.0	50.0	58.0	58.0	1289 bp	0.5	59.8
trnF(gaa)-rpl19_p2	AGCCAATTGATAGCAGAGGC	ACAACGAAAGACAAGCCCAT	50.0	45.0	58.0	58.0	1694 bp	0.5	59.7
trnF(gaa)-rpl19_p3	AGCCAATTGATAGCAGAGGC	GTAAAGTGCGAGTGTTTGCC	50.0	50.0	58.0	58.0	1177 bp	0.5	59.6
trnF(gaa)-rpl19_p4	AGCCAATTGATAGCAGAGGC	TGAGCAATAGCAGCAGGAAG	50.0	50.0	58.0	58.0	1140 bp	0.5	59.6
trnF(gaa)-rpl19_p5	AGCCAATTGATAGCAGAGGC	CTGAGATGGCCTGCTTACAG	50.0	55.0	58.0	58.0	1397 bp	0.5	59.4