Hotspot

trnI(gau)-orf340

Genome · 159417-160247 · 831 bp

Back to Streptosarcina

Pi 0.327

MarkerSeek score 80.50

Primer available yes

Label trnI(gau)-orf340

Feature Metadata

label_name: trnI(gau)-orf340
feature_type: igs
region: Genome
length_bp: 831
variable_sites: 267
pi: 0.327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI(gau)-orf340_p1	TTGAAGCCTACGTCATGCTC	GCAATTGAGAGCGAAGGAGA	50.0	50.0	58.0	58.0	1842 bp	0.5	59.8
trnI(gau)-orf340_p2	TTGAAGCCTACGTCATGCTC	CTCAGCTTCGTAGTCGCTTT	50.0	50.0	58.0	58.0	1523 bp	0.5	59.8
trnI(gau)-orf340_p3	TTGAAGCCTACGTCATGCTC	CGAGATGCTGTTCGTGAAGA	50.0	50.0	58.0	58.0	1749 bp	0.5	59.8
trnI(gau)-orf340_p4	AAGCCTACGTCATGCTCTTG	GCAATTGAGAGCGAAGGAGA	50.0	50.0	58.0	58.0	1839 bp	0.5	59.7
trnI(gau)-orf340_p5	CTTGAAGCCTACGTCATGCT	GCAATTGAGAGCGAAGGAGA	50.0	50.0	58.0	58.0	1843 bp	0.5	59.7