Hotspot

minD-ndhB

Genome · 144084-145956 · 1873 bp

Back to Streptosarcina

Pi 0.194

MarkerSeek score 82.30

Primer available yes

Label minD-ndhB

Feature Metadata

label_name: minD-ndhB
feature_type: igs
region: Genome
length_bp: 1873
variable_sites: 358
pi: 0.194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
minD-ndhB_p1	ATCTTCTCTCTGTCCACGGT	CACTCTCGTTCGTAGTCTGC	50.0	55.0	57.8	58.1	1939 bp	0.5	57.3
minD-ndhB_p2	ATCTTCTCTCTGTCCACGGT	TTGGTGTGAGCCCATTCATC	50.0	50.0	57.8	58.2	1973 bp	0.5	56.9
minD-ndhB_p3	ATCTTCTCTCTGTCCACGGT	AGGACACTCTCGTTCGTAGT	50.0	50.0	57.8	57.8	1943 bp	0.5	56.8
minD-ndhB_p4	ATCTTCTCTCTGTCCACGGT	GTAGTCTGCCTCTCTCGTGA	50.0	55.0	57.8	58.3	1928 bp	0.5	56.1
minD-ndhB_p5	ATCTTCTCTCTGTCCACGGT	ATTCATCTCCCTAGCCCAGG	50.0	55.0	57.8	58.3	1960 bp	0.5	56.1