Hotspot

ycf1-chlN

Genome · 138697-139302 · 606 bp

Back to Streptosarcina

Pi 0.0723
MarkerSeek score 79.30
Primer available yes
Label ycf1-chlN

Feature Metadata

label_name
ycf1-chlN
feature_type
igs
region
Genome
length_bp
606
variable_sites
42
pi
0.0723
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-chlN_p1 TGTTGTTCGGTTCCACACAT GGTTCCCATGCCTAGGATTG 45.0 55.0 58.0 58.0 1326-1350 bp 1.0 84.1
ycf1-chlN_p2 GAGGTGGGCGTTAGGTTTAG GGTTCCCATGCCTAGGATTG 55.0 55.0 58.0 58.0 1523-1547 bp 1.0 84.0
ycf1-chlN_p3 GAGGTGGGCGTTAGGTTTAG GGACAGCTACCACACTCATG 55.0 55.0 58.0 58.0 1875-1899 bp 1.0 83.4
ycf1-chlN_p4 GAGGTGGGCGTTAGGTTTAG ATGCTCCGTGTTTGAGATCC 55.0 50.0 58.0 58.0 1778-1802 bp 1.0 83.4
ycf1-chlN_p5 GAGGTGGGCGTTAGGTTTAG GGAGCAGATATGGAGGGGAT 55.0 55.0 58.0 58.0 1733-1757 bp 1.0 83.4