Hotspot

minD-ccsA

Genome · 116826-118070 · 1245 bp

Back to Streptosarcina

Pi 0.394

MarkerSeek score 88.00

Primer available yes

Label minD-ccsA

Feature Metadata

label_name: minD-ccsA
feature_type: igs
region: Genome
length_bp: 1245
variable_sites: 425
pi: 0.394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 88.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
minD-ccsA_p1	TCTTCTCGCTCTGGGAATCT	TCAATTGGTTGGGCAAAGGA	50.0	45.0	57.8	57.9	1395-1407 bp	1.0	90.0
minD-ccsA_p2	TCTTCTCGCTCTGGGAATCT	AATTGGTTGGGCAAAGGACT	50.0	45.0	57.8	57.9	1393-1405 bp	1.0	90.0
minD-ccsA_p3	TCTTCTCGCTCTGGGAATCT	ATTGGTTGGGCAAAGGACTT	50.0	45.0	57.8	57.9	1392-1404 bp	1.0	90.0
minD-ccsA_p4	TCTTCTCGCTCTGGGAATCT	TGGGCAAAGGACTTCATAGC	50.0	50.0	57.8	57.9	1386-1398 bp	1.0	89.8
minD-ccsA_p5	TCTTCTCGCTCTGGGAATCT	CAATTGGTTGGGCAAAGGAC	50.0	50.0	57.8	57.8	1394-1406 bp	1.0	89.4