Hotspot

rpl19-trnF(gaa)

Genome · 93160-96312 · 3153 bp

Back to Streptosarcina

Pi 0.454

MarkerSeek score 76.00

Primer available yes

Label rpl19-trnF(gaa)

Feature Metadata

label_name: rpl19-trnF(gaa)
feature_type: igs
region: Genome
length_bp: 3153
variable_sites: 794
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl19-trnF(gaa)_p1	AAGTGGGGCTTGCATAAACA	GGCAAACACTCGCACTTTAC	45.0	50.0	58.0	58.0	1075 bp	0.5	59.7
rpl19-trnF(gaa)_p2	AGTGGGGCTTGCATAAACAA	GGCAAACACTCGCACTTTAC	45.0	50.0	58.0	58.0	1074 bp	0.5	59.7
rpl19-trnF(gaa)_p3	AAGTGGGGCTTGCATAAACA	CTTCCTGCTGCTATTGCTCA	45.0	50.0	58.0	58.0	1112 bp	0.5	59.7
rpl19-trnF(gaa)_p4	AGTGGGGCTTGCATAAACAA	CTTCCTGCTGCTATTGCTCA	45.0	50.0	58.0	58.0	1111 bp	0.5	59.7
rpl19-trnF(gaa)_p5	GAGCGCATCCGATATCCAAT	GGCAAACACTCGCACTTTAC	50.0	50.0	58.0	58.0	928 bp	0.5	59.6