Hotspot

trnQ(uug)-chlB

Genome · 73389-73722 · 334 bp

Back to Streptosarcina

Pi 0.0817
MarkerSeek score 80.30
Primer available yes
Label trnQ(uug)-chlB

Feature Metadata

label_name
trnQ(uug)-chlB
feature_type
igs
region
Genome
length_bp
334
variable_sites
25
pi
0.0817
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ(uug)-chlB_p1 AGCGGTAAAACACGTGACTT GTCTCCTAAAGGTGCATGCA 45.0 50.0 58.1 57.9 472-500 bp 1.0 86.8
trnQ(uug)-chlB_p2 AAGCGGTAAAACACGTGACT GTCTCCTAAAGGTGCATGCA 45.0 50.0 58.1 57.9 473-501 bp 1.0 86.7
trnQ(uug)-chlB_p3 GACTCATGCGTAGGAGGTTC GTCTCCTAAAGGTGCATGCA 55.0 50.0 57.8 57.9 451-479 bp 1.0 85.7
trnQ(uug)-chlB_p4 AAGCGGTAAAACACGTGACT AGTCGTCTCCTAAAGGTGCA 45.0 50.0 58.1 58.4 477-505 bp 1.0 84.7
trnQ(uug)-chlB_p5 AGCGGTAAAACACGTGACTT AGTCGTCTCCTAAAGGTGCA 45.0 50.0 58.1 58.4 476-504 bp 1.0 84.7