Hotspot

rps8

LSC · 82542-82940 · 399 bp

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Pi 0.00251

MarkerSeek score 46.10

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 1
pi: 0.00251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	ATAACAGGAGGGGAGGATGG	GGGTACCGATAATTATAGTTCACC	55.0	41.7	57.9	56.5	447 bp	1.0	40.2
rps8_p2	GGATAACAGGAGGGGAGGAT	GGGTACCGATAATTATAGTTCACC	55.0	41.7	57.6	56.5	449 bp	1.0	40.2
rps8_p3	AGGATAACAGGAGGGGAGGA	GGGTACCGATAATTATAGTTCACC	55.0	41.7	58.7	56.5	450 bp	1.0	40.2
rps8_p4	TAACAGGAGGGGAGGATGGA	GGGTACCGATAATTATAGTTCACC	55.0	41.7	59.0	56.5	446 bp	1.0	40.2
rps8_p5	TAGGATAACAGGAGGGGAGG	GGGTACCGATAATTATAGTTCACC	55.0	41.7	56.9	56.5	451 bp	1.0	40.2