Hotspot

rpl32

SSC · 116110-116271 · 162 bp

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Pi 0.483

MarkerSeek score 76.60

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 162
variable_sites: 72
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	AAAAGCCCACATACGACGAA	GTTCCCGATGGGTTAGCAAA	45.0	50.0	57.8	58.2	747-767 bp	1.0	88.6
rpl32_p2	AAAAGCCCACATACGACGAA	CAATCCCACCCATCAGTTCC	45.0	55.0	57.8	58.2	762-782 bp	1.0	88.1
rpl32_p3	AAAAGCCCACATACGACGAA	CCAATCCCACCCATCAGTTC	45.0	55.0	57.8	58.2	763-783 bp	1.0	88.1
rpl32_p4	AAAAGCCCACATACGACGAA	TTTCACCATGGCGGCTTATT	45.0	45.0	57.8	58.2	1476-2159 bp	1.0	83.2
rpl32_p5	AAAGCCCACATACGACGAAT	TTTCACCATGGCGGCTTATT	45.0	45.0	57.6	58.2	1475-2158 bp	1.0	81.4