Hotspot

ndhF-rpl32

SSC · 115672-116109 · 438 bp

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Pi 0.0118

MarkerSeek score 50.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 438
variable_sites: 5
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACACATTCCCACCAGTTCC	GTTCCCGATGGGTTAGCAAA	50.0	50.0	58.0	58.2	1093-1113 bp	1.0	86.9
ndhF-rpl32_p2	CATTCCCACCAGTTCCCAAA	GTTCCCGATGGGTTAGCAAA	50.0	50.0	58.0	58.2	1089-1109 bp	1.0	86.9
ndhF-rpl32_p3	AACACATTCCCACCAGTTCC	GCCCAGTGGAAACCGATTTA	50.0	50.0	58.0	58.2	1034-1852 bp	1.0	83.6
ndhF-rpl32_p4	CATTCCCACCAGTTCCCAAA	GCCCAGTGGAAACCGATTTA	50.0	50.0	58.0	58.2	1030-1848 bp	1.0	83.6
ndhF-rpl32_p5	GAAGAGGGAATTGTGCGGAT	GCCCAGTGGAAACCGATTTA	50.0	50.0	58.0	58.2	1301-2119 bp	1.0	82.5