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petA-psbJ

LSC · 64818-66058 · 1241 bp

Back to Streptopus

Pi 0.00249

MarkerSeek score 62.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 3
pi: 0.00249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.7	58.3	1368-1383 bp	1.0	74.3
petA-psbJ_p2	GATCCATTACGCGTCCAAGG	GGTTCATATTCCGGGTTGGG	55.0	55.0	58.8	58.3	1379-1394 bp	1.0	73.4
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.7	57.6	1364-1379 bp	1.0	73.3
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.7	57.6	1367-1382 bp	1.0	73.3
petA-psbJ_p5	GATCCATTACGCGTCCAAGG	CATATTCCGGGTTGGGTTCA	55.0	50.0	58.8	57.6	1375-1390 bp	1.0	72.5