Hotspot

ycf4-cemA

LSC · 62177-62912 · 736 bp

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Pi 0.00272

MarkerSeek score 60.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 2
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTAGCCTATTTCTTGCGCGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.0	58.1	831-855 bp	1.0	79.4
ycf4-cemA_p2	AAAAGCTGCCGAATTAGCCT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.2	58.1	844-868 bp	1.0	78.3
ycf4-cemA_p3	TTAGCCTATTTCTTGCGCGT	GAGAGAGAGATCCACCAGGG	45.0	60.0	58.0	58.3	837-861 bp	1.0	77.7
ycf4-cemA_p4	CCTTGACTGGCACTGATGAG	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.3	58.1	890-914 bp	1.0	77.4
ycf4-cemA_p5	TTAGCCTATTTCTTGCGCGT	TTCGGATTGCTGGGTATTCC	45.0	50.0	58.0	57.7	895-919 bp	1.0	77.3