Hotspot

ndhC-trnV-UAC

LSC · 51610-52840 · 1231 bp

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Pi 0.00412
MarkerSeek score 62.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1231
variable_sites
5
pi
0.00412
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCCTTCACTAATCGGTGGTG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1369-1378 bp 1.0 79.6
ndhC-trnV-UAC_p2 ATCGGTGGTGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1359-1368 bp 1.0 78.9
ndhC-trnV-UAC_p3 GTGGTGCTAAAACTCCGGAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1355-1364 bp 1.0 78.9
ndhC-trnV-UAC_p4 CCCTTCACTAATCGGTGGTG AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1451-1460 bp 1.0 77.3
ndhC-trnV-UAC_p5 CCCTTCACTAATCGGTGGTG GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.0 57.5 1368-1377 bp 1.0 76.8