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trnF-GAA-ndhJ

LSC · 49049-49918 · 870 bp

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Pi 0.00464

MarkerSeek score 62.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 4
pi: 0.00464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	980-1003 bp	1.0	79.5
trnF-GAA-ndhJ_p2	AAAGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	1023-1046 bp	1.0	78.8
trnF-GAA-ndhJ_p3	AAGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	1022-1045 bp	1.0	78.8
trnF-GAA-ndhJ_p4	ACATAGATCAAGTGCTCCGC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	1060-1083 bp	1.0	78.0
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	1011-1034 bp	1.0	77.4