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psbM-trnD-GUC

LSC · 29917-30930 · 1014 bp

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Pi 0.00198

MarkerSeek score 60.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 2
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GCGGTAGGAACTAGAATGAACA	CCTCGCTGGGATTGTAGTTC	45.5	55.0	57.9	58.1	1148-1154 bp	1.0	63.1
psbM-trnD-GUC_p2	GCGGTAGGAACTAGAATGAACA	AGTTCAATCGGTCAGAGCAC	45.5	50.0	57.9	57.9	1133-1139 bp	1.0	62.9
psbM-trnD-GUC_p3	GCGGTAGGAACTAGAATGAACA	TACACCTCGCTGGGATTGTA	45.5	50.0	57.9	58.1	1152-1158 bp	1.0	62.4
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1136-1142 bp	1.0	62.3
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1121-1127 bp	1.0	62.0