Hotspot

ycf1

SSC · 123078-128573 · 5496 bp

Back to Streptocarpus

Pi 0.00238

MarkerSeek score 46.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5496
variable_sites: 38
pi: 0.00238
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGCAAAAATTCTCTATTTATTCTGGGA	TGAACGATCTGACTCATAGAACA	29.6	39.1	57.5	57.1	266-274 bp	1.0	43.8
ycf1_p2	GCAAAAATTCTCTATTTATTCTGGGA	TGAACGATCTGACTCATAGAACA	30.8	39.1	56.3	57.1	265-273 bp	1.0	43.8
ycf1_p3	AGCAAAAATTCTCTATTTATTCTGGG	TGAACGATCTGACTCATAGAACA	30.8	39.1	56.3	57.1	266-274 bp	1.0	43.8
ycf1_p4	AGCAAAAATTCTCTATTTATTCTGGGA	AAGATGAACGATCTGACTCA	29.6	40.0	57.5	53.8	270-278 bp	1.0	43.8
ycf1_p5	AGCAAAAATTCTCTATTTATTCTGGGA	TGAACGATCTGACTCATAGAACAA	29.6	37.5	57.5	57.7	266-274 bp	1.0	43.8