Hotspot

rps16

LSC · 5095-6971 · 1877 bp

Back to Streptocarpus

Pi 0.00316
MarkerSeek score 52.50
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1877
variable_sites
17
pi
0.00316
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TATGAATCGAGCAGGAAGGG ACTCAAGCCTATCCCTATGT 50.0 45.0 56.8 55.0 2078-2096 bp 1.0 47.1
rps16_p2 TATGAATCGAGCAGGAAGGG ACTCAAGCCTATCCCTATGTT 50.0 42.9 56.8 55.8 2078-2096 bp 1.0 45.9
rps16_p3 ATGAATCGAGCAGGAAGGGG ACTCAAGCCTATCCCTATGT 55.0 45.0 59.5 55.0 2077-2095 bp 1.0 44.6
rps16_p4 ATGAATCGAGCAGGAAGGGG ACTCAAGCCTATCCCTATGTT 55.0 42.9 59.5 55.8 2077-2095 bp 1.0 43.3
rps16_p5 ATATGAATCGAGCAGGAAGGG ACTCAAGCCTATCCCTATGT 47.6 45.0 57.3 55.0 2079-2097 bp 1.0 43.3