Hotspot

rpoC2

LSC · 16685-20830 · 4146 bp

Back to Streptocarpus

Pi 0.00125

MarkerSeek score 55.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4146
variable_sites: 15
pi: 0.00125
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACTAGGTGTGATTCGTTGAACA	TTCATTCCCGAGGAAGTACA	40.9	45.0	57.7	55.9	309 bp	1.0	45.1
rpoC2_p2	ACTAGGTGTGATTCGTTGAACA	TTTCATTCCCGAGGAAGTACA	40.9	42.9	57.7	56.7	310 bp	1.0	43.4
rpoC2_p3	ACTAGGTGTGATTCGTTGAACA	TCATTCCCGAGGAAGTACAT	40.9	45.0	57.7	55.6	308 bp	1.0	43.2
rpoC2_p4	AGGTGTGATTCGTTGAACAT	TTCATTCCCGAGGAAGTACA	40.0	45.0	55.3	55.9	306 bp	1.0	42.1
rpoC2_p5	ACTAGGTGTGATTCGTTGAACA	TTCATTCCCGAGGAAGTACAT	40.9	42.9	57.7	56.4	309 bp	1.0	41.6