Hotspot

atpA

LSC · 10267-11790 · 1524 bp

Back to Streptocarpus

Pi 0

MarkerSeek score 25.60

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	2353-2483 bp	1.0	80.3
atpA_p2	CAAAATGCTGAACCGACGTC	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	2386-2516 bp	1.0	80.1
atpA_p3	CGCTACCCGCTTATTTCCTT	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.1	58.0	2320-2450 bp	1.0	80.1
atpA_p4	AGCCTTCCAAGCTAACGATG	GGAAGCCGATGAGTTTAGGG	50.0	55.0	58.0	58.0	2129-2259 bp	1.0	80.1
atpA_p5	CCTAGCCTTCCAAGCTAACG	GTAATTGAGCGTGAGAGCCA	55.0	50.0	58.1	58.0	2356-2486 bp	1.0	80.0