Hotspot

rpl20-rps12

LSC · 68980-69790 · 811 bp

Back to Streptocarpus

Pi 0.00164

MarkerSeek score 55.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 4
pi: 0.00164
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTGTTCTACGTCTCCGAGC	AAATGTCACGAAATCCCCCG	50.0	50.0	57.7	58.5	919-928 bp	1.0	74.1
rpl20-rps12_p2	TGTTCTACGTCTCCGAGCTA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.6	58.5	917-926 bp	1.0	73.7
rpl20-rps12_p3	TTGTTCTACGTCTCCGAGCT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.5	58.5	918-927 bp	1.0	73.1
rpl20-rps12_p4	TTTGTTCTACGTCTCCGAGC	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.7	58.7	882-891 bp	1.0	72.6
rpl20-rps12_p5	TGTTCTACGTCTCCGAGCTA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.6	58.7	880-889 bp	1.0	72.2