Hotspot

ycf4-cemA

LSC · 61036-61532 · 497 bp

Back to Streptocarpus

Pi 0.00335

MarkerSeek score 31.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 5
pi: 0.00335
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTTGCGCGTACCAATTGAAG	GAGAGAGAAATCCAACAGGACA	45.0	45.5	57.7	57.5	602 bp	1.0	57.7
ycf4-cemA_p2	TTTGCGCGTACCAATTGAAG	TGAGAGAGAAATCCAACAGGAC	45.0	45.5	57.7	57.5	603 bp	1.0	57.7
ycf4-cemA_p3	TTTGCGCGTACCAATTGAAG	TCCAACAGGACAAAAACACT	45.0	40.0	57.7	55.3	592 bp	1.0	56.5
ycf4-cemA_p4	TTTGCGCGTACCAATTGAAG	AGAGAGAAATCCAACAGGACA	45.0	42.9	57.7	56.3	601 bp	1.0	56.3
ycf4-cemA_p5	TTTGCGCGTACCAATTGAAG	AGAGAGAAATCCAACAGGACAA	45.0	40.9	57.7	57.0	601 bp	1.0	54.2