Hotspot

trnS-UGA-psbZ

LSC · 35923-36243 · 321 bp

Back to Streptocarpus

Pi 0.0027

MarkerSeek score 41.30

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 321
variable_sites: 2
pi: 0.0027
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	GAGAAGCAAATACGACGGGT	55.0	50.0	58.0	58.0	429-430 bp	1.0	80.3
trnS-UGA-psbZ_p2	GTTTTCAAGACCGGGGCTAT	GAGAAGCAAATACGACGGGT	50.0	50.0	57.9	58.0	449-450 bp	1.0	79.5
trnS-UGA-psbZ_p3	CAACCACTCAGCCATCTCTC	CGACGGGTACGCTAATCAAT	55.0	50.0	58.0	57.9	417-418 bp	1.0	79.4
trnS-UGA-psbZ_p4	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	440-441 bp	1.0	78.7
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	CGACGGGTACGCTAATCAAT	50.0	50.0	57.9	57.9	437-438 bp	1.0	78.5