Hotspot

atpA-atpF

LSC · 11791-11859 · 69 bp

Back to Streptocarpus

Pi 0.0435

MarkerSeek score 78.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 9
pi: 0.0435
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CACTTGAAGTACGGTACCGG	ACATGTTGGGAGCGATGAAA	55.0	45.0	58.0	57.8	207-210 bp	1.0	82.5
atpA-atpF_p2	CCACTTGAAGTACGGTACCG	ACATGTTGGGAGCGATGAAA	55.0	45.0	58.0	57.8	208-211 bp	1.0	82.5
atpA-atpF_p3	CAATACCATCGCCCACTTGA	ACATGTTGGGAGCGATGAAA	50.0	45.0	58.0	57.8	220-223 bp	1.0	82.1
atpA-atpF_p4	CACTTGAAGTACGGTACCGG	GACATGTTGGGAGCGATGAA	55.0	50.0	58.0	58.3	208-211 bp	1.0	82.0
atpA-atpF_p5	CCACTTGAAGTACGGTACCG	GACATGTTGGGAGCGATGAA	55.0	50.0	58.0	58.3	209-212 bp	1.0	82.0