Hotspot

trnS-CGA-trnR-UCU

LSC · 10062-10117 · 56 bp

Back to Streptocarpus

Pi 0.0238

MarkerSeek score 53.70

Primer available yes

Label trnS-CGA-trnR-UCU

Feature Metadata

label_name: trnS-CGA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 4
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-CGA-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	137-269 bp	1.0	65.5
trnS-CGA-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	140-272 bp	1.0	65.3
trnS-CGA-trnR-UCU_p3	CAAAATGCTGAACCGACGTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	170-302 bp	1.0	65.1
trnS-CGA-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	143-275 bp	1.0	64.8
trnS-CGA-trnR-UCU_p5	CAAAATGCTGAACCGACGTC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	176-308 bp	1.0	64.4