Hotspot

trnK-UUU-rps16

LSC · 4290-5094 · 805 bp

Back to Streptocarpus

Pi 0.00216
MarkerSeek score 64.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
805
variable_sites
5
pi
0.00216
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GTCGGGGTCTTCCAAACTTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 975-1006 bp 1.0 81.0
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 893-924 bp 1.0 80.1
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 892-923 bp 1.0 80.1
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 907-938 bp 1.0 79.3
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 902-933 bp 1.0 79.2