Hotspot

atpB

LSC · 56269-57747 · 1479 bp

Back to Stranvaesia

Pi 0.00135

MarkerSeek score 39.20

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 3
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	ACAATTCGATTCGGGGTCAG	CCGATCAACTTAACTTGTTTTGC	50.0	39.1	58.0	57.2	1624 bp	1.0	49.5
atpB_p2	CAATTCGATTCGGGGTCAGT	CCGATCAACTTAACTTGTTTTGC	50.0	39.1	58.0	57.2	1623 bp	1.0	49.5
atpB_p3	TCGGGGTCAGTACACAAAGA	CCGATCAACTTAACTTGTTTTGC	50.0	39.1	58.3	57.2	1614 bp	1.0	47.4
atpB_p4	TTCGATTCGGGGTCAGTACA	CCGATCAACTTAACTTGTTTTGC	50.0	39.1	58.5	57.2	1620 bp	1.0	46.1
atpB_p5	AACAATTCGATTCGGGGTCA	CCGATCAACTTAACTTGTTTTGC	45.0	39.1	57.5	57.2	1625 bp	1.0	45.8