Hotspot

ndhF-rpl32

SSC · 116526-117599 · 1074 bp

Back to Stranvaesia

Pi 0.00124

MarkerSeek score 60.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1074
variable_sites: 2
pi: 0.00124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	40.0	45.0	57.0	57.8	1269-1270 bp	1.0	70.7
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	40.0	45.0	57.0	57.8	1267-1268 bp	1.0	70.4
ndhF-rpl32_p3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	40.0	55.0	57.0	58.3	1256-1257 bp	1.0	69.4
ndhF-rpl32_p4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	40.0	45.0	57.0	57.6	1266-1267 bp	1.0	68.5
ndhF-rpl32_p5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	38.1	45.0	57.7	57.8	1270-1271 bp	1.0	68.5