Hotspot

psaJ-rpl33

LSC · 70704-71157 · 454 bp

Back to Stranvaesia

Pi 0.00587

MarkerSeek score 60.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 4
pi: 0.00587
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	555-561 bp	1.0	70.9
psaJ-rpl33_p2	GTTCGGGTCTTTAGCAGGTT	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	600-606 bp	1.0	70.7
psaJ-rpl33_p3	TCCGGATGCGTTGATATTCC	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	582-588 bp	1.0	70.7
psaJ-rpl33_p4	GTTCGGGTCTTTAGCAGGTT	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	627-633 bp	1.0	70.5
psaJ-rpl33_p5	TGGCACCGGTAGTAAGTACT	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	625-631 bp	1.0	70.4