Hotspot

petN-psbM

LSC · 30159-31441 · 1283 bp

Back to Stranvaesia

Pi 0.00727

MarkerSeek score 63.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1283
variable_sites: 14
pi: 0.00727
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.0	57.6	1389-1407 bp	1.0	77.1
petN-psbM_p2	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1332-1350 bp	1.0	76.4
petN-psbM_p3	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1331-1349 bp	1.0	76.4
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.8	57.6	1330-1348 bp	1.0	75.9
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	AAAACAGTCAGCCAAGGTGA	60.0	45.0	58.2	57.6	1327-1345 bp	1.0	75.6