Hotspot

trnH-GUG-psbA

LSC · 125-395 · 271 bp

Back to Stranvaesia

Pi 0.0172

MarkerSeek score 57.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 6
pi: 0.0172
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	394-402 bp	1.0	80.9
trnH-GUG-psbA_p2	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	397-405 bp	1.0	80.8
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	333-341 bp	1.0	80.8
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	347-355 bp	1.0	80.7
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	346-354 bp	1.0	80.7