Hotspot

rps16

LSC · 4445-5554 · 1110 bp

Back to Stipagrostis

Pi 0.0042

MarkerSeek score 58.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1110
variable_sites: 7
pi: 0.0042
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CTGGTGACCCTCTCCTACTT	TCTCGATACCACTTTGCAATGA	55.0	40.9	57.8	57.8	1196 bp	1.0	60.9
rps16_p2	CTGGTGACCCTCTCCTACTT	CTCGATACCACTTTGCAATGA	55.0	42.9	57.8	56.3	1195 bp	1.0	57.3
rps16_p3	CTGGTGACCCTCTCCTACTT	TCTCGATACCACTTTGCAATG	55.0	42.9	57.8	56.3	1196 bp	1.0	57.3
rps16_p4	TGGTGACCCTCTCCTACTTT	TCTCGATACCACTTTGCAATGA	50.0	40.9	57.3	57.8	1195 bp	1.0	57.1
rps16_p5	TACTGGTGACCCTCTCCTAC	TCTCGATACCACTTTGCAATGA	55.0	40.9	57.2	57.8	1198 bp	1.0	56.3