Hotspot

rpl16

LSC · 77219-78727 · 1509 bp

Back to Stipagrostis

Pi 0.0084

MarkerSeek score 53.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1509
variable_sites: 19
pi: 0.0084
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTAGAGGCATTTGGGGCAAT	AATTGCAGGTCGTCTAGCAG	50.0	50.0	57.9	58.0	2075-2078 bp	1.0	80.3
rpl16_p2	CTCGGCAATTGCACCAAAAA	AATTGCAGGTCGTCTAGCAG	45.0	50.0	58.1	58.0	2230-2233 bp	1.0	79.7
rpl16_p3	TTTCTAGAGGCATTTGGGGC	AATTGCAGGTCGTCTAGCAG	50.0	50.0	57.9	58.0	2078-2081 bp	1.0	79.7
rpl16_p4	GCTCCACTGTTATCCGCTAC	AATTGCAGGTCGTCTAGCAG	55.0	50.0	58.1	58.0	1962-1965 bp	1.0	79.7
rpl16_p5	CTCAATTCCTCGGCAATTGC	AATTGCAGGTCGTCTAGCAG	50.0	50.0	57.8	58.0	2238-2241 bp	1.0	79.2