Hotspot

ndhD

SSC · 107730-109232 · 1503 bp

Back to Stipagrostis

Pi 0.00266

MarkerSeek score 47.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 6
pi: 0.00266
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCAACTCTTTTGTGCGGATC	TTTAGGGCCTGAAACAACCC	50.0	50.0	58.0	58.0	2381-2382 bp	1.0	80.5
ndhD_p2	GCAACTCTTTTGTGCGGATC	AAACCATTGCTTCGTCTGGT	50.0	45.0	58.0	58.0	2696-2697 bp	1.0	80.4
ndhD_p3	GCAACTCTTTTGTGCGGATC	TCTTGGCTCTTTTCACGCTT	50.0	45.0	58.0	58.0	2770-2771 bp	1.0	80.2
ndhD_p4	GCAACTCTTTTGTGCGGATC	TGGCTCTTTTCACGCTTTCT	50.0	45.0	58.0	58.0	2767-2768 bp	1.0	80.2
ndhD_p5	GCAACTCTTTTGTGCGGATC	GGGCTTTTCTGGTCAAAACG	50.0	50.0	58.0	57.9	2262-2263 bp	1.0	79.4