Hotspot

psbT-psbN

LSC · 70218-70270 · 53 bp

Back to Stipagrostis

Pi 0.0629

MarkerSeek score 74.30

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 53
variable_sites: 5
pi: 0.0629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGCCTAAGGTTCCGACTAAA	TCGCCATCTCCATATCTGGT	50.0	50.0	57.3	58.0	192 bp	1.0	76.7
psbT-psbN_p2	TTCTTCCGAGAACCGCCTAA	TCGCCATCTCCATATCTGGT	50.0	50.0	58.7	58.0	205 bp	1.0	75.9
psbT-psbN_p3	TATCTTCTTCCGAGAACCGC	TCGCCATCTCCATATCTGGT	50.0	50.0	57.1	58.0	209 bp	1.0	74.9
psbT-psbN_p4	CGCCTAAGGTTCCGACTAAA	GTCGCCATCTCCATATCTGG	50.0	55.0	57.3	57.6	193 bp	1.0	73.8
psbT-psbN_p5	TCTTCCGAGAACCGCCTAA	TCGCCATCTCCATATCTGGT	52.6	50.0	58.0	58.0	204 bp	1.0	73.6