Hotspot

petA-psbJ

LSC · 60130-61038 · 909 bp

Back to Stipagrostis

Pi 0.0117
MarkerSeek score 57.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
909
variable_sites
16
pi
0.0117
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAAAAGGTTCAATTGTACGA CGGGTCGTATTCTGGATTGG 33.3 55.0 53.7 58.1 976-1020 bp 1.0 41.5
petA-psbJ_p2 TGAAAAGGTTCAATTGTACGA GGGTCGTATTCTGGATTGGG 33.3 55.0 53.7 57.7 975-1019 bp 1.0 41.5
petA-psbJ_p3 TGAAAAGGTTCAATTGTACGAA CGGGTCGTATTCTGGATTGG 31.8 55.0 54.5 58.1 976-1020 bp 1.0 41.5
petA-psbJ_p4 TTGAAAAGGTTCAATTGTACGA CGGGTCGTATTCTGGATTGG 31.8 55.0 54.5 58.1 977-1021 bp 1.0 41.5
petA-psbJ_p5 AGAAACAGTTTGAAAAGGTTCA CGGGTCGTATTCTGGATTGG 31.8 55.0 54.6 58.1 986-1030 bp 1.0 41.4