Hotspot

rbcL-psaI

LSC · 55967-56949 · 983 bp

Back to Stipagrostis

Pi 0.013

MarkerSeek score 56.30

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 17
pi: 0.013
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TACAAGCTCGTAACGAAGGC	TAACTGGAAACCCCAACTGC	50.0	50.0	58.0	58.0	1675-1754 bp	1.0	84.6
rbcL-psaI_p2	GGCTTCAGGGGGTATTCATG	TAACTGGAAACCCCAACTGC	55.0	50.0	58.0	58.0	1827-1906 bp	1.0	84.2
rbcL-psaI_p3	TACAAGCTCGTAACGAAGGC	AATCGGTCATAACCACTGCC	50.0	50.0	58.0	58.0	1831-1910 bp	1.0	84.0
rbcL-psaI_p4	GTTATACCGGTGGCTTCAGG	TAACTGGAAACCCCAACTGC	55.0	50.0	58.1	58.0	1838-1917 bp	1.0	83.9
rbcL-psaI_p5	GGCTTCAGGGGGTATTCATG	AATCGGTCATAACCACTGCC	55.0	50.0	58.0	58.0	1983-2062 bp	1.0	83.7