Hotspot

atpB-rbcL

LSC · 53739-54535 · 797 bp

Back to Stipagrostis

Pi 0.00418

MarkerSeek score 58.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 5
pi: 0.00418
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATCCAGCACGGGTCCAATA	CTTGGTTTCGTACTCCGGAG	50.0	55.0	58.2	58.0	990-991 bp	1.0	78.8
atpB-rbcL_p2	CCAGCACGGGTCCAATAATT	CTTGGTTTCGTACTCCGGAG	50.0	55.0	58.2	58.0	987-988 bp	1.0	78.5
atpB-rbcL_p3	TATCCAGCACGGGTCCAATA	GCTTGAAATCCAACACCTGC	50.0	50.0	58.2	57.9	938-939 bp	1.0	78.3
atpB-rbcL_p4	CCCGGGGGAAAAGTGATATC	CTTGGTTTCGTACTCCGGAG	55.0	55.0	57.7	58.0	1006-1007 bp	1.0	78.2
atpB-rbcL_p5	CCGGGGGAAAAGTGATATCC	CTTGGTTTCGTACTCCGGAG	55.0	55.0	57.7	58.0	1005-1006 bp	1.0	78.2